Plate IV.475 · Publications
Pitchfork and Gprasp2 Target Smoothened to the Primary Cilium for Hedgehog Pathway Activation
Article in PLOS ONE, 22 February 2016.
Abstract
The seven-transmembrane receptor Smoothened (Smo) activates all Hedgehog (Hh) signaling by translocation into the primary cilia (PC), but how this is regulated is not well understood. Here we show that Pitchfork (Pifo) and the G protein-coupled receptor associated sorting protein 2 (Gprasp2) are essential components of an Hh induced ciliary targeting complex able to regulate Smo translocation to the PC. Depletion of Pifo or Gprasp2 leads to failure of Smo translocation to the PC and lack of Hh target gene activation. Together, our results identify a novel protein complex that is regulated by Hh signaling and required for Smo ciliary trafficking and Hh pathway activation.
Cite
@article{jung2016pitchfork,
title = {{Pitchfork and Gprasp2 Target Smoothened to the Primary Cilium for Hedgehog Pathway Activation}},
author = {Jung, Bomi and Padula, Daniela and Burtscher, Ingo and Landerer, Cedric and Lutter, Dominik and Theis, Fabian and Messias, Ana C. and Geerlof, Arie and Sattler, Michael and Kremmer, Elisabeth and Boldt, Karsten and Ueffing, Marius and Lickert, Heiko},
journal = {PLOS ONE},
publisher = {Public Library of Science (PLoS)},
year = {2016},
month = feb,
doi = {10.1371/journal.pone.0149477},
url = {https://doi.org/10.1371/journal.pone.0149477},
pmid = {26901434},
pmcid = {PMC4763541}
}