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Plate IV.518 · Publications

Quantification of regenerative potential in primary human mammary epithelial cells

Article in Development, 1 January 2015.

Open accessLung, Epithelial Cells

  1. Jelena R. Linnemann
  2. Haruko Miura
  3. Lisa K. Meixner
  4. Martin Irmler
  5. Uwe J. Kloos
  6. Benjamin Hirschi
  7. Harald S. Bartsch
  8. Steffen Sass
  9. Johannes Beckers
  10. Fabian J. Theis
  11. Christian Gabka
  12. Karl Sotlar
  13. Christina H. Scheel

Abstract

We present an organoid regeneration assay in which freshly isolated human mammary epithelial cells are cultured in adherent or floating collagen gels, corresponding to a rigid or compliant matrix environment. In both conditions, luminal progenitors form spheres, whereas basal cells generate branched ductal structures. In compliant but not rigid collagen gels, branching ducts form alveoli at their tips, express basal and luminal markers at correct positions, and display contractility, which is required for alveologenesis. Thereby, branched structures generated in compliant collagen gels resemble terminal ductal-lobular units (TDLUs), the functional units of the mammary gland. Using the membrane metallo-endopeptidase CD10 as a surface marker enriches for TDLU formation and reveals the presence of stromal cells within the CD49f(hi)/EpCAM(-) population. In summary, we describe a defined in vitro assay system to quantify cells with regenerative potential and systematically investigate their interaction with the physical environment at distinct steps of morphogenesis.

Cite

@article{linnemann2015quantification,
  title = {{Quantification of regenerative potential in primary human mammary epithelial cells}},
  author = {Linnemann, Jelena R. and Miura, Haruko and Meixner, Lisa K. and Irmler, Martin and Kloos, Uwe J. and Hirschi, Benjamin and Bartsch, Harald S. and Sass, Steffen and Beckers, Johannes and Theis, Fabian J. and Gabka, Christian and Sotlar, Karl and Scheel, Christina H.},
  journal = {Development},
  publisher = {The Company of Biologists},
  year = {2015},
  month = jan,
  doi = {10.1242/dev.123554},
  url = {https://doi.org/10.1242/dev.123554},
  pmid = {26071498},
  pmcid = {PMC4582177}
}
.bib file