Plate IV.367 · Publications
A cellular census of healthy lung and asthmatic airway wall identifies novel cell states in health and disease
Preprint in bioRxiv, 23 January 2019.
Abstract
Human lungs enable efficient gas exchange, and form an interface with the environment which depends on mucosal immunity for protection against infectious agents. Tightly controlled interactions between structural and immune cells are required to maintain lung homeostasis. Here, we use single cell transcriptomics to chart the cellular landscape of upper and lower airways and lung parenchyma in health. We report location-dependent airway epithelial cell states, and a novel subset of tissue-resident memory T cells. In lower airways of asthma patients, mucous cell hyperplasia is shown to stem from a novel mucous ciliated cell state, as well as goblet cell hyperplasia. We report presence of pathogenic effector Th2 cells in asthma, and find evidence for type-2 cytokines in maintaining the altered epithelial cell states. Unbiased analysis of cell-cell interactions identify a shift from airway structural cell communication in health to a Th2-dominated interactome in asthma.
Cite
@misc{vieirabraga2019cellular_e359,
title = {{A cellular census of healthy lung and asthmatic airway wall identifies novel cell states in health and disease}},
author = {Vieira Braga, F.A. and Kar, G. and Berg, M. and Carpaij, O.A. and Polanski, K. and Simon, L.M. and Brouwer, S. and Gomes, T. and Hesse, L. and Jiang, J. and Fasouli, E.S. and Efremova, M. and Vento-Tormo, R. and Affleck, K. and Palit, S. and Strzelecka, P. and Firth, H.V. and Mahbubani, K.t.a. and Cvejic, A. and Meyer, K.B. and Saeb-Parsy, K. and Luinge, M. and Brandsma, C.-A. and Timens, W. and Angelidis, I. and Strunz, M. and Koppelman, G.H. and van Oosterhout, A.J. and Schiller, H.B. and Theis, F.J. and van den Berge, M. and Nawijn, M.C. and Teichmann, S.A.},
howpublished = {bioRxiv},
publisher = {openRxiv},
year = {2019},
month = jan,
doi = {10.1101/527408},
url = {https://doi.org/10.1101/527408},
note = {Preprint}
}