Plate IV.81 · Publications
Im-seq: Multimodal droplet barcoding enables high-throughput linking of single-cell imaging and gene expression
Preprint in bioRxiv, 1 September 2025.
Abstract
Understanding sequence-phenotype relationships in biology requires large-scale datasets that provide one-to-one mapping of sequence to phenotype. Here, we address this challenge by introducing multimodal droplet barcoding, enabling individual microfluidic droplets to be tracked across both optical and sequencing measurements. We demonstrate the power of this approach through multimodal single-cell analysis. We pair imaging with gene expression profiling of individual cells, identifying genes that correlate with phenotypic features.
Cite
@misc{xu2025im,
title = {{Im-seq: Multimodal droplet barcoding enables high-throughput linking of single-cell imaging and gene expression}},
author = {Xu, Catherine K and Meisl, Georg and Moshkov, Nikita and Schmacke, Niklas A and Goda, Karolis and Shkarin, Alexey and Schlögel, Maximilian F and Diebold, Sandra and Schweitzer, Christine and Ekici, Arif and Knowles, Tuomas PJ and Theis, Fabian J and Mazutis, Linas and Guck, Jochen},
howpublished = {bioRxiv},
publisher = {openRxiv},
year = {2025},
month = sep,
doi = {10.1101/2025.09.01.672798},
url = {https://doi.org/10.1101/2025.09.01.672798},
note = {Preprint}
}