Plate IV.349 · Publications
A cellular census of human lungs identifies novel cell states in health and in asthma
Article in Nature Medicine, 17 June 2019.
Abstract
Human lungs enable efficient gas exchange and form an interface with the environment, which depends on mucosal immunity for protection against infectious agents. Tightly controlled interactions between structural and immune cells are required to maintain lung homeostasis. Here, we use single-cell transcriptomics to chart the cellular landscape of upper and lower airways and lung parenchyma in healthy lungs, and lower airways in asthmatic lungs. We report location-dependent airway epithelial cell states and a novel subset of tissue-resident memory T cells. In the lower airways of patients with asthma, mucous cell hyperplasia is shown to stem from a novel mucous ciliated cell state, as well as goblet cell hyperplasia. We report the presence of pathogenic effector type 2 helper T cells (TH2) in asthmatic lungs and find evidence for type 2 cytokines in maintaining the altered epithelial cell states. Unbiased analysis of cell-cell interactions identifies a shift from airway structural cell communication in healthy lungs to a TH2-dominated interactome in asthmatic lungs.
Cite
@article{vieirabraga2019cellular_ffb5,
title = {{A cellular census of human lungs identifies novel cell states in health and in asthma}},
author = {Vieira Braga, Felipe A. and Kar, Gozde and Berg, Marijn and Carpaij, Orestes A. and Polanski, Krzysztof and Simon, Lukas M. and Brouwer, Sharon and Gomes, Tomás and Hesse, Laura and Jiang, Jian and Fasouli, Eirini S. and Efremova, Mirjana and Vento-Tormo, Roser and Talavera-López, Carlos and Jonker, Marnix R. and Affleck, Karen and Palit, Subarna and Strzelecka, Paulina M. and Firth, Helen V. and Mahbubani, Krishnaa T. and Cvejic, Ana and Meyer, Kerstin B. and Saeb-Parsy, Kourosh and Luinge, Marjan and Brandsma, Corry-Anke and Timens, Wim and Angelidis, Ilias and Strunz, Maximilian and Koppelman, Gerard H. and van Oosterhout, Antoon J. and Schiller, Herbert B. and Theis, Fabian J. and van den Berge, Maarten and Nawijn, Martijn C. and Teichmann, Sarah A.},
journal = {Nature Medicine},
publisher = {Springer Science and Business Media LLC},
year = {2019},
month = jun,
doi = {10.1038/s41591-019-0468-5},
url = {https://doi.org/10.1038/s41591-019-0468-5},
pmid = {31209336}
}