Plate IV.582 · Publications
Identification and Quantification of 1-Hydroxybutene-2-yl Mercapturic Acid in Human Urine by UPLC- HILIC-MS/MS as a Novel Biomarker for 1,3-Butadiene Exposure
Article in Chemical Research in Toxicology, 27 July 2012.
Abstract
1,3-Butadiene (BD) is a Class 1 carcinogen present at workplaces, in polluted air, in automobile exhaust, and in tobacco smoke. 2-Hydroxybutene-1-yl mercapturic acid (2-MHBMA) is a urinary metabolite often measured as a biomarker for exposure to BD. Here, we show for the first time that an additional MHBMA isomer is present at significant amounts in human urine, 1-hydroxybutene-2-yl mercapturic acid (1-MHBMA). For its quantification, a highly sensitive UPLC-HILIC-MS/MS method was developed and validated. Analyzing urinary samples of 183 volunteers, we demonstrate that 1-MHBMA is a novel and potentially more reliable biomarker for BD exposure than the commonly analyzed 2-MHBMA.
Cite
@article{sterz2012identification,
title = {{Identification and Quantification of 1-Hydroxybutene-2-yl Mercapturic Acid in Human Urine by UPLC- HILIC-MS/MS as a Novel Biomarker for 1,3-Butadiene Exposure}},
author = {Sterz, Katharina and Scherer, Gerhard and Krumsiek, Jan and Theis, Fabian J. and Ecker, Josef},
journal = {Chemical Research in Toxicology},
publisher = {American Chemical Society (ACS)},
year = {2012},
month = jul,
doi = {10.1021/tx3002862},
url = {https://doi.org/10.1021/tx3002862},
pmid = {22817647}
}