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Plate IV.174 · Publications

Single-cell characterization of neovascularization using hiPSC-derived endothelial cells in a 3D microenvironment

Article in Stem Cell Reports, October 2023.

Open accessStem Cells, Lineage

  1. Simon Rosowski
  2. Caroline Remmert
  3. Maren Marder
  4. Misao Akishiba
  5. Judith Bushe
  6. Annette Feuchtinger
  7. Alina Platen
  8. Siegfried Ussar
  9. Fabian Theis
  10. Sandra Wiedenmann
  11. Matthias Meier

Abstract

The formation of vascular structures is fundamental for in vitro tissue engineering. Vascularization can enable the nutrient supply within larger structures and increase transplantation efficiency. We differentiated human induced pluripotent stem cells toward endothelial cells in 3D suspension culture. To investigate in vitro neovascularization and various 3D microenvironmental approaches, we designed a comprehensive single-cell transcriptomic study. Time-resolved single-cell transcriptomics of the endothelial and co-evolving mural cells gave insights into cell type development, stability, and plasticity. Transfer to a 3D hydrogel microenvironment induced neovascularization and facilitated tracing of migrating, coalescing, and tubulogenic endothelial cell states. During maturation, we monitored two pericyte subtypes evolving mural cells. Profiling cell-cell interactions between pericytes and endothelial cells revealed angiogenic signals during tubulogenesis. In silico discovered ligands were tested for their capability to attract endothelial cells. Our data, analyses, and results provide an in vitro roadmap to guide vascularization in future tissue engineering.

Versions2

  1. PublishedStem Cell Reports10.1016/j.stemcr.2023.08.008
  2. PreprintbioRxiv10.1101/2022.02.15.480506

Cite

@article{rosowski2023single,
  title = {{Single-cell characterization of neovascularization using hiPSC-derived endothelial cells in a 3D microenvironment}},
  author = {Rosowski, Simon and Remmert, Caroline and Marder, Maren and Akishiba, Misao and Bushe, Judith and Feuchtinger, Annette and Platen, Alina and Ussar, Siegfried and Theis, Fabian and Wiedenmann, Sandra and Meier, Matthias},
  journal = {Stem Cell Reports},
  publisher = {Elsevier BV},
  year = {2023},
  month = oct,
  doi = {10.1016/j.stemcr.2023.08.008},
  url = {https://doi.org/10.1016/j.stemcr.2023.08.008},
  pmid = {37714147},
  pmcid = {PMC10656300}
}
.bib file